Sylwia Karoń

The doctoral thesis aims to create new designs of multi-parameter immunosensors for diagnosing viral infections. The goal of the study is to develop a methodology for generating receptor layers based on hybridizing single-stranded DNA anchors (DDI – DNA-directed immobilization) and using it for immunodiagnostics and profiling the body’s response to viral infections. Such a layer will be created by selecting optimal conditions for immobilization and receptor layer interactions. The DDI technique allows targeted immobilization and, consequently, the creation of reproducible protein microarrays, distinguished by their compatibility with microfluidic systems.