Karolina Porycka

The goal of my thesis is to develop a new class of microsystems for the rapid detection of specific antibodies as biomarkers of immune response in blood serum. These microsystems are expected to demonstrate significant advantages over current ELISA-based assay formats, particularly in terms of sensitivity and specificity within a biomedical context. My research focuses on testing novel strategies for creating labeled immunocomplexes, using bifunctional conjugates of peroxidase mimetics for controlled magnetic separation of labeled immunocomplexes from serum, and developing in-flow signal-reading methods. The results of this research will contribute to the creation of a simple, integrated Point-of-Care diagnostic microsystem capable of detecting a wide range of bacterial infections.